Known pathogenic gene variants and new candidates detected in Sudden Unexpected Infant Death using Whole Genome Sequencing

  • Angela M Bard ,
  • Lindsay V Clark ,
  • ,
  • Kimberly A Aldinger ,
  • Andrew Timms ,
  • Lely A Quina ,
  • ,
  • David Jardine ,
  • Elisabeth A Haas ,
  • Tatiana M Becker ,
  • Chelsea M Pagan ,
  • Avni Santani ,
  • Diego Martinez ,
  • Soumitra Barua ,
  • Zakkary McNutt ,
  • Addie Nesbitt ,
  • Edwin A Mitchell ,
  • Jan-Marino Ramirez

Purpose

To gain insights into potential genetic factors contributing to the infant’s vulnerability to Sudden Unexpected Infant Death (SUID).

Methods

Whole Genome Sequencing (WGS) was performed on 145 infants that succumbed to SUID, and 576 healthy adults. Variants were filtered by gnomAD allele frequencies and predictions of functional consequences.

Results

Variants of interest were identified in 86 genes, 63.4% of our cohort. Seventy-one of these have been previously associated with SIDS/SUID/SUDP. Forty-three can be characterized as cardiac genes and are related to cardiomyopathies, arrhythmias, and other conditions. Variants in 22 genes were associated with neurologic functions. Variants were also found in 13 genes reported to be pathogenic for various systemic disorders. Variants in eight genes are implicated in the response to hypoxia and the regulation of reactive oxygen species (ROS) and have not been previously described in SIDS/SUID/SUDP. Seventy-two infants met the triple risk hypothesis criteria (Figure 1).

Conclusion

Our study confirms and further expands the list of genetic variants associated with SUID. The abundance of genes associated with heart disease and the discovery of variants associated with the redox metabolism have important mechanistic implications for the pathophysiology of SUID.